Fundación Jiménez Díaz-Biobank

The Biobank of the Jiménez Díaz Foundation (B-FJD) is a non-profit infrastructure of high strategic value, conceived as a decentralized transversal platform to support the IIS-FJD, with the capacity to collect samples, generate cellular and animal models and provision of services to the research groups of the Institute itself, the hospital and external users. Since its constitution in 2009, one of the main objectives of the B-FJD has been the integration of the biobank’s own activities into the activity of healthcare services, with all its members being staff with shared dedication to healthcare. This has made it possible for the Unit to currently have personnel hired by the IIS-FJD and the HUFJD involved in the collection, registration of biological samples and provision of services. The same occurs with the new satellite centers of the biobank, where the collection and processing of samples is assumed as part of the healthcare activity.

Services

Service Portfolio
1. Transfer of samples:
Paraffin block sections in eppendorf
Sections of paraffin block in slide
Paraffin block sections on treated slides
HE staining of paraffin block on untreated slides
OCT frozen tissue sections in eppendorf
OCT Frozen Tissue Sections on Slide
OCT frozen tissue sections on treated slides
HE staining of OCT frozen tissue
Plasma Aliquot
Serum Aliquot

2. Processing and analysis services: processing and analysis services can be applied to samples belonging to the biobank, in requests for transfer and processing or to external samples in requests for processing only:
DNA extraction from frozen tissue sample (OCT sections)
DNA extraction from FFPE tissue sample
DNA extraction from whole blood samples
RNA extraction from frozen tissue sample (OCT sections)
RNA extraction from FFPE tissue sample
RNA extraction from whole blood samples
Quantification of nucleic acid samples (1 sample)
Quality determination of nucleic acid samples (purity and integrity)
Separation of blood mononuclear cells (10 ml)
Obtaining aliquots of plasma or serum (500 μl aliquots)
Embedding samples in formalin and paraffin
Hematoxylin-Eosin staining
Histochemistry staining
Immunohistochemistry staining
Optimization of immunohistochemistry stains
Image digitization
Tissue Micro Array (TMA) Construction

3. Storage of collections: integration as collections of samples outside the organizational scope of the biobank, but protected under the infrastructure of the biobank in quality criteria.
Storage of aliquots in liquid nitrogen in liquid phase
Storage of aliquots in a -80ºC freezer
Storage of 1 box of 81 positions in liquid nitrogen in liquid phase
Storage of 1 box of 81 positions in a -80ºC freezer
Creation and management of new collections